Fig 1: Corroboration of Akirin2–IP interactions in HL60 cellsSome of the previously identified Akirin2 IPs [14] were selected for analysis of Akirin2–IP interactions in human HL60 cells. (A) Representation of the pull-down experiment using COOH-Myc-tagged human Akirin2 with c-Myc magnetic beads and HL60 cells lysate. (B) Akirin2 was incubated with HL60 lysate and immunoprecipitated with c-Myc magnetic beads specific for the Akirin2 protein Myc-tag. HL60 lysate and beads incubated only with HL60 lysate were used as positive (C+) and negative (C-) controls, respectively. PBS and recAkirin2 were also used as secondary controls. Akirin2-IPs were detected by mouse or rabbit antibodies specific for each protein and visualized by a dot blot analysis with anti-mouse or anti-rabbit secondary antibodies. (C) Dot blot areas of Akirin2–IPs dots were measured using ImageJ program and compared with the dots of SNRNP70, a protein used as negative control due to its low interaction with Akirin2, by Student’s t test with unequal variance (*P<0.05; n=2 biological replicates).